TCEB1 Polyclonal antibody

TCEB1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12450-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

SIII p15, Elongin-C, Elongin C, Elongin 15 kDa subunit, EloC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12450-1-AP targets TCEB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原 TCEB1 fusion protein Ag3087 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)
别名 SIII p15, Elongin-C, Elongin C, Elongin 15 kDa subunit, EloC
计算分子量 112 aa, 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC013809
基因名称 TCEB1
Gene ID (NCBI) 6921
RRIDAB_2201139
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15369
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCEB1, also named as RNA polymerase II transcription factor SIII subunit C, belongs to the SKP1 family. It mediates the ubiquitination of ECS-E3 ubiquitin-protein ligase complexes. TCEB1 is overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3. TCEB1 also promotes invasion of prostate cancer cells (PMID:18844214).

实验方案

Product Specific Protocols
WB protocol for TCEB1 antibody 12450-1-APDownload protocol
IHC protocol for TCEB1 antibody 12450-1-APDownload protocol
IF protocol for TCEB1 antibody 12450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation

Authors - Magda Wąchalska
humanWB

Cell Death Differ

CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptosis through promoting p14ARF degradation.

Authors - Pingzhao Zhang
humanIHC

Am J Pathol

A 12-gene expression signature is associated with aggressive histological in prostate cancer: SEC14L1 and TCEB1 genes are potential markers of progression.

Authors - Agell Laia L
ratWB

Evid Based Complement Alternat Med

Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism.

Authors - Chong-Chong Guo
humanWB

bioRxiv

The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation

Authors - Magda Wąchalska
humanWB

bioRxiv

Allosteric degraders induce CRL5 ASB8 mediated degradation of XPO1

Authors - Casey Elizabeth Hudman-Wing
Loading...