验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Jurkat cells, THP-1 cells, Raji cells, HEK-293 cells, HepG2 cells, K-562 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HCT 116 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67787-1-Ig targets TK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | TK1 fusion protein Ag8265 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | thymidine kinase 1, soluble |
别名 | Tk1b, Tk1a, TK 2, TK 1, EC:2.7.1.21 |
计算分子量 | 25 kDa |
观测分子量 | 25 kDa |
GenBank蛋白编号 | BC007986 |
基因名称 | TK1 |
Gene ID (NCBI) | 7083 |
RRID | AB_2918551 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P04183 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker(PMID:21178264).
实验方案
Product Specific Protocols | |
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WB protocol for TK1 antibody 67787-1-Ig | Download protocol |
IHC protocol for TK1 antibody 67787-1-Ig | Download protocol |
IF protocol for TK1 antibody 67787-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |