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TK1 Monoclonal antibody

TK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1B1C2

Cat no : 67787-1-Ig

Print datasheet

Synonyms

Tk1b, Tk1a, TK 2, TK 1, EC:2.7.1.21

验证数据展示

  • WB analysis using 67787-1-Ig

    WB analysis using 67787-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of human colon cancer using 67787-1-Ig

    IHC staining of human colon cancer using 67787-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67787-1-Ig (TK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TK1 antibody included
  • IHC staining of human colon cancer using 67787-1-Ig

    IHC staining of human colon cancer using 67787-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67787-1-Ig (TK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TK1 antibody included
  • WB analysis using 67787-1-Ig

    WB analysis using 67787-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 67787-1-Ig

    WB analysis using 67787-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human tonsillitis using 67787-1-Ig

    IHC staining of human tonsillitis using 67787-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67787-1-Ig (TK1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TK1 antibody included
  • IF Staining of HCT 116 using 67787-1-Ig

    IF Staining of HCT 116 using 67787-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using TK1 antibody (67787-1-Ig, Clone: 1B1C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, THP-1 cells, Raji cells, HEK-293 cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHCT 116 cells
Planning an IHC experiment? We recommend our IHCeasy TK1 Ready-To-Use IHC Kit. TK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67787-1-Ig targets TK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen TK1 fusion protein Ag8265 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name thymidine kinase 1, soluble
Synonyms Tk1b, Tk1a, TK 2, TK 1, EC:2.7.1.21
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC007986
Gene Symbol TK1
Gene ID (NCBI) 7083
RRIDAB_2918551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04183
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker(PMID:21178264).

实验方案

Product Specific Protocols
WB protocol for TK1 antibody 67787-1-IgDownload protocol
IHC protocol for TK1 antibody 67787-1-IgDownload protocol
IF protocol for TK1 antibody 67787-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Cell Death Dis

m5C-dependent cross-regulation between nuclear reader ALYREF and writer NSUN2 promotes urothelial bladder cancer malignancy through facilitating RABL6/TK1 mRNAs splicing and stabilization

Authors - Ning Wang
View Article