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TK1 Monoclonal antibody, PBS Only

TK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B1C2

Cat no : 67787-1-PBS

Print datasheet

Synonyms

thymidine kinase 1, soluble, Thymidine kinase, cytosolic, TK1, TK2

验证数据展示

  • WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)

    WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67787-1-Ig (same clone as 67787-1-PBS)

    IHC staining of human colon cancer using 67787-1-Ig (same clone as 67787-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67787-1-Ig (TK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TK1 antibody included
  • IHC staining of human colon cancer using 67787-1-Ig (same clone as 67787-1-PBS)

    IHC staining of human colon cancer using 67787-1-Ig (same clone as 67787-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67787-1-Ig (TK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TK1 antibody included
  • WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)

    WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

  • WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)

    WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

  • IF Staining of HCT 116 using 67787-1-Ig (same clone as 67787-1-PBS)

    IF Staining of HCT 116 using 67787-1-Ig (same clone as 67787-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using TK1 antibody (67787-1-Ig, Clone: 1B1C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TK1 Ready-To-Use IHC Kit. TK1 primary antibody included.

产品信息

67787-1-PBS targets TK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen TK1 fusion protein Ag8265 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name thymidine kinase 1, soluble
Synonyms thymidine kinase 1, soluble, Thymidine kinase, cytosolic, TK1, TK2
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC007986
Gene Symbol TK1
Gene ID (NCBI) 7083
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04183
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker(PMID:21178264).