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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TLR2 Monoclonal antibody

TLR2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C1D3

Cat no : CL488-66645

Print datasheet

Synonyms

CD282, TIL4, TLR2, toll like receptor 2

验证数据展示

  • IF Staining of THP-1 using CL488-66645

    IF Staining of THP-1 using CL488-66645

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 488 TLR2 antibody (CL488-66645, Clone: 1C1D3 ) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inTHP-1 cells
For other applications, we recommend the unconjugated version of this antibody, 66645-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66645 targets TLR2 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen TLR2 fusion protein Ag25626 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name toll-like receptor 2
Synonyms CD282, TIL4, TLR2, toll like receptor 2
Calculated Molecular Weight 784 aa, 90 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC033756
Gene Symbol TLR2
Gene ID (NCBI) 7097
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60603
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TLR2, also known as CD282, is a member of the Toll-like receptor (TLR) family. TLR receptors are type I integral transmembrane glycoproteins which play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs) and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TLR2 antibody CL488-66645Download protocol
FC protocol for CL Plus 488 TLR2 antibody CL488-66645Download protocol
Standard Protocols
Click here to view our Standard Protocols