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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of THP-1 using CL488-66570

    FC experiment of THP-1 using CL488-66570

    1X10^6 THP-1 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human TLR5 (CL488-66570, Clone:4B3D2) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated TLR5 Monoclonal antibody

TLR5 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66570

产品说明书

CloneNo. 4B3D2

宿主/亚型

Mouse / IgG1

种属反应性

Human, Pig, Mouse, Rat

应用

FC (Intra)

别名

Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3, toll like receptor 5, TIL3, SLEB1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inTHP-1 cells
For other applications, we recommend the unconjugated version of this antibody, 66570-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66570 targets TLR5 in FC (Intra) applications and shows reactivity with Human, Pig, Mouse, Rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Pig, Mouse, Rat
免疫原 TLR5 fusion protein Ag13840 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 toll-like receptor 5
别名 Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3, toll like receptor 5, TIL3, SLEB1
计算分子量 858 aa, 98 kDa
观测分子量 100 kDa
GenBank蛋白编号BC109119
基因名称 TLR5
Gene ID (NCBI) 7100
RRIDAB_3084259
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO60602
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TLR5 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR5 is known to recognize bacterial flagellin from invading mobile bacteria. TLR5 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Mutations in TLR5 gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TLR5 antibody CL488-66570Download protocol
Standard Protocols
Click here to view our Standard Protocols