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TMEM59 Polyclonal antibody

TMEM59 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24134-1-AP

Print datasheet

Synonyms

C1orf8, HSPC001, Liver membrane bound protein, TMEM59, transmembrane protein 59, UNQ169/PRO195

验证数据展示

  • IHC staining of human breast cancer using 24134-1-AP

    IHC staining of human breast cancer using 24134-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24134-1-AP (TMEM59 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 24134-1-AP

    IHC staining of human breast cancer using 24134-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24134-1-AP (TMEM59 Antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of MCF-7 using 24134-1-AP

    WB analysis of MCF-7 using 24134-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24134-1-AP (TMEM59 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 24134-1-AP

    IF Staining of HepG2 using 24134-1-AP

    Immunofluorescent analysis of HepG2 cells using 24134-1-AP (TMEM59 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

24134-1-AP targets TMEM59 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen TMEM59 fusion protein Ag21150 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 59
Synonyms C1orf8, HSPC001, Liver membrane bound protein, TMEM59, transmembrane protein 59, UNQ169/PRO195
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC016374
Gene Symbol TMEM59
Gene ID (NCBI) 9528
RRIDAB_2879439
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXS4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM59 is a regulator of autophagy in response to bacterial infection. Overexpression of this protein has been found to promote apoptosis in a glioma cell line. Alternative splicing of TMEM59 results in multiple transcript variants.

实验方案

Product Specific Protocols
WB protocol for TMEM59 antibody 24134-1-APDownload protocol
IHC protocol for TMEM59 antibody 24134-1-APDownload protocol
IF protocol for TMEM59 antibody 24134-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.

Authors - Julian Nüchel
View Article
humanWB

J Extracell Vesicles

A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.

Authors - Charles P Hinzman
View Article