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  • Featured Product
  • KD/KO Validated

TNNC1 Polyclonal antibody

TNNC1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13504-1-AP

Print datasheet

Synonyms

TNNC, TN-C, TNC, TN C

验证数据展示

  • WB analysis of mouse skeletal muscle using 13504-1-AP

    WB analysis of mouse skeletal muscle using 13504-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13504-1-AP (TNNC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 13504-1-AP

    IHC staining of human skeletal muscle using 13504-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13504-1-AP (TNNC1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skeletal muscle using 13504-1-AP

    IHC staining of human skeletal muscle using 13504-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13504-1-AP (TNNC1 Antibody) at dilution of 1:200 (under 40x lens).

  • IP experiment of mouse heart using 13504-1-AP

    IP experiment of mouse heart using 13504-1-AP

    IP result of anti-TNNC1 (IP:13504-1-AP, 4ug; Detection:13504-1-AP 1:600) with mouse heart tissue lysate 4400ug.

  • IF Staining of U2OS using 13504-1-AP

    IF Staining of U2OS using 13504-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TNNC1 antibody (13504-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of U2OS using 13504-1-AP

    FC experiment of U2OS using 13504-1-AP

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug TNNC1 Polyclonal antibody (13504-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, human stomach tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13504-1-AP targets TNNC1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen TNNC1 fusion protein Ag4339 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name troponin C type 1 (slow)
Synonyms TNNC, TN-C, TNC, TN C
Calculated Molecular Weight 161 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC030244
Gene Symbol TNNC1
Gene ID (NCBI) 7134
RRIDAB_2256271
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP63316
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. TnC plays an important role in excitation-contraction coupling and is the primary target of compounds such as levosimendan that augment Ca2+ responsiveness of the thin filament.

实验方案

Product Specific Protocols
WB protocol for TNNC1 antibody 13504-1-APDownload protocol
IHC protocol for TNNC1 antibody 13504-1-APDownload protocol
IF protocol for TNNC1 antibody 13504-1-APDownload protocol
IP protocol for TNNC1 antibody 13504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Calcium-dependent FAK/CREB/TNNC1 signalling mediates the effect of stromal MFAP5 on ovarian cancer metastatic potential.

Authors - Cecilia S Leung
  • KD Validated
View Article
ratWB

J Proteome Res

iTRAQ-based proteomic analysis reveals recovery of impaired mitochondrial function in ischemic myocardium by Shenmai formula.

Authors - Yi Wang
View Article
mouseIHC,IF

Front Cardiovasc Med

GSK-J4, a Specific Histone Lysine Demethylase 6A Inhibitor, Ameliorates Lipotoxicity to Cardiomyocytes via Preserving H3K27 Methylation and Reducing Ferroptosis.

Authors - Kai Xu
View Article
mouseWB

Mol Cell Biol

MUNC, an Enhancer RNA Upstream from the MYOD Gene, Induces a Subgroup of Myogenic Transcripts in trans Independently of MyoD.

Authors - Magdalena A Cichewicz
View Article
humanWB

Onco Targets Ther

LukS-PV Inhibits Hepatocellular Carcinoma Cells Migration via the TNNC1/PI3K/AKT Axis.

Authors - Fan Ma
View Article
humanWB

Med Sci Monit

TNNC1 Reduced Gemcitabine Sensitivity of Nonsmall-Cell Lung Cancer by Increasing Autophagy.

Authors - Xian Ye
View Article