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TRBP Polyclonal antibody

TRBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15753-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

TARBP2, TRBP1, Trans-activation-responsive RNA-binding protein, TAR RNA-binding protein 2, RISC-loading complex subunit TARBP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, mouse liver tissue, mouse spleen tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15753-1-AP targets TRBP in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF, IP, RIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 TRBP fusion protein Ag8255 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TAR (HIV-1) RNA binding protein 2
别名 TARBP2, TRBP1, Trans-activation-responsive RNA-binding protein, TAR RNA-binding protein 2, RISC-loading complex subunit TARBP2
计算分子量 39 kDa
观测分子量 43-46 kDa
GenBank蛋白编号BC005860
基因名称 TRBP
Gene ID (NCBI) 6895
RRIDAB_2200485
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15633
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43-46 kDa, are expressed in human cells. (PMID: 16142218)

实验方案

Product Specific Protocols
WB protocol for TRBP antibody 15753-1-APDownload protocol
IHC protocol for TRBP antibody 15753-1-APDownload protocol
IF protocol for TRBP antibody 15753-1-APDownload protocol
IP protocol for TRBP antibody 15753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Mol Cell

Nuclear TARBP2 Drives Oncogenic Dysregulation of RNA Splicing and Decay.

Authors - Lisa Fish
  • KD Validated
mouseWB

Mol Cell

A Rapid Induction Mechanism for Lin28a in Trophic Responses.

Authors - Alexandra M Amen
humanWB

Nat Commun

Hypoxia regulates overall mRNA homeostasis by inducing Met1-linked linear ubiquitination of AGO2 in cancer cells.

Authors - Hailong Zhang
humanWB

Nat Commun

SUMOylation of TARBP2 regulates miRNA/siRNA efficiency.

Authors - Cheng Chen
  • KD Validated
humanWB,IF

Nucleic Acids Res

S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells.

Authors - Matthew J Warner
  • KD Validated
humanWB

J Med Chem

Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.

Authors - Zongyuan Zhou
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