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验证数据展示

  • IF Staining of HeLa using CL488-15753

    IF Staining of HeLa using CL488-15753

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TRBP antibody (CL488-15753) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-15753

    FC experiment of HeLa using CL488-15753

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 TRBP Polyclonal Antibody (CL488-15753)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TRBP Polyclonal antibody

TRBP Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-15753

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

TARBP2, TRBP1, Trans-activation-responsive RNA-binding protein, TAR RNA-binding protein 2, RISC-loading complex subunit TARBP2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 15753-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15753 targets TRBP in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen TRBP fusion protein Ag8255 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TAR (HIV-1) RNA binding protein 2
Synonyms TARBP2, TRBP1, Trans-activation-responsive RNA-binding protein, TAR RNA-binding protein 2, RISC-loading complex subunit TARBP2
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 43-46 kDa
GenBank Accession NumberBC005860
Gene Symbol TRBP
Gene ID (NCBI) 6895
RRIDAB_3672639
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15633
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43-46 kDa, are expressed in human cells. (PMID: 16142218)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TRBP antibody CL488-15753Download protocol
FC protocol for CL Plus 488 TRBP antibody CL488-15753Download protocol
Standard Protocols
Click here to view our Standard Protocols