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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TRIM32 Polyclonal antibody

TRIM32 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10326

Print datasheet

Synonyms

tripartite motif containing 32, RING-type E3 ubiquitin transferase TRIM32, HT2A, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM32

验证数据展示

  • FC experiment of A431 using CL488-10326

    FC experiment of A431 using CL488-10326

    1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human TRIM32 (CL488-10326) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy TRIM32 Ready-To-Use IHC Kit. TRIM32 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10326-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10326 targets TRIM32 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen TRIM32 fusion protein Ag0401 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 32
Synonyms tripartite motif containing 32, RING-type E3 ubiquitin transferase TRIM32, HT2A, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM32
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 65-72 kDa
GenBank Accession NumberBC003154
Gene Symbol TRIM32
Gene ID (NCBI) 22954
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13049
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TRIM32 antibody CL488-10326Download protocol
Standard Protocols
Click here to view our Standard Protocols