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验证数据展示

  • FC experiment of A431 using CL488-10326

    FC experiment of A431 using CL488-10326

    1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human TRIM32 (CL488-10326) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • KD/KO验证

CoraLite® Plus 488-conjugated TRIM32 Polyclonal antibody

TRIM32 Polyclonal Antibody for FC (Intra)
Cat No. CL488-10326

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

tripartite motif containing 32, RING-type E3 ubiquitin transferase TRIM32, HT2A, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM32

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy TRIM32 Ready-To-Use IHC Kit. TRIM32 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10326-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10326 targets TRIM32 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 TRIM32 fusion protein Ag0401 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 32
别名 tripartite motif containing 32, RING-type E3 ubiquitin transferase TRIM32, HT2A, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM32
计算分子量 72 kDa
观测分子量 65-72 kDa
GenBank蛋白编号BC003154
基因名称 TRIM32
Gene ID (NCBI) 22954
RRIDAB_3672438
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13049
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TRIM32 antibody CL488-10326Download protocol
Standard Protocols
Click here to view our Standard Protocols