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验证数据展示

  • IF Staining of HeLa using CL555-15047

    IF Staining of HeLa using CL555-15047

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 Thymidylate synthase antibody (CL555-15047) at dilution of 1:100.

  • FC experiment of HeLa using CL555-15047

    FC experiment of HeLa using CL555-15047

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Thymidylate synthase (CL555-15047) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®555-conjugated Thymidylate synthase Polyclonal antibody

Thymidylate synthase Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-15047

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

TYMS, TMS, thymidylate synthetase, OK/SW-cl.29, HsT422

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TYMS Ready-To-Use IHC Kit. TYMS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15047-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-15047 targets Thymidylate synthase in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Thymidylate synthase fusion protein Ag7027 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thymidylate synthetase
别名 TYMS, TMS, thymidylate synthetase, OK/SW-cl.29, HsT422
计算分子量 36 kDa
观测分子量 32-36 kDa
GenBank蛋白编号BC002567
基因名称 TYMS
Gene ID (NCBI) 7298
RRIDAB_2919622
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04818
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.

实验方案

Product Specific Protocols
IF protocol for CL555 Thymidylate synthase antibody CL555-15047Download protocol
FC protocol for CL555 Thymidylate synthase antibody CL555-15047Download protocol
Standard Protocols
Click here to view our Standard Protocols