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CoraLite®555-conjugated Thymidylate synthase Polyclonal antibody
Thymidylate synthase Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy TYMS Ready-To-Use IHC Kit. TYMS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15047-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL555-15047 targets Thymidylate synthase in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Thymidylate synthase fusion protein Ag7027 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | thymidylate synthetase |
Synonyms | TYMS, TMS, thymidylate synthetase, OK/SW-cl.29, HsT422 |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 32-36 kDa |
GenBank Accession Number | BC002567 |
Gene Symbol | TYMS |
Gene ID (NCBI) | 7298 |
RRID | AB_2919622 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 557 nm / 570 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P04818 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.
实验方案
Product Specific Protocols | |
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IF protocol for CL555 Thymidylate synthase antibody CL555-15047 | Download protocol |
FC protocol for CL555 Thymidylate synthase antibody CL555-15047 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |