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UCH-L1/PGP9.5 Polyclonal antibody
UCH-L1/PGP9.5 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA and More (2)
Conjugate
Unconjugated
124
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, NCI-H1299 cells, SH-SY5Y cells, Neuro-2a cells, C6 cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | mouse brain tissue, human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Positive IF/ICC detected in | A549 cells, mouse brain tissue |
Positive FC (Intra) detected in | C6 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14730-1-AP targets UCH-L1/PGP9.5 in WB, IHC, IF/ICC, IF-P, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, IF, CoIP, ELISA |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, canine, monkey, non-human primate |
Immunogen | UCH-L1/PGP9.5 fusion protein Ag6490 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) |
Synonyms | PGP9.5, UCHL1, UCHL1/PGP9.5, PGP9/5, PGP 9.5 |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 27 kDa |
GenBank Accession Number | BC000332 |
Gene Symbol | UCHL1 |
Gene ID (NCBI) | 7345 |
RRID | AB_2210497 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P09936 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for UCH-L1/PGP9.5 antibody 14730-1-AP | Download protocol |
IHC protocol for UCH-L1/PGP9.5 antibody 14730-1-AP | Download protocol |
IF protocol for UCH-L1/PGP9.5 antibody 14730-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Stem Cell Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency. | ||