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- KD/KO Validated
CoraLite®594-conjugated UCH-L1/PGP9.5 Polyclonal antibody
UCH-L1/PGP9.5 Polyclonal Antibody for IF-P, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P, FC (Intra) and More (1)
Conjugate
CoraLite®594 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | Y79 cells |
Planning an IHC experiment? We recommend our IHCeasy UCHL1 Ready-To-Use IHC Kit. UCHL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14730-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL594-14730 targets UCH-L1/PGP9.5 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF-P, FC (Intra) Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Immunogen | UCH-L1/PGP9.5 fusion protein Ag6490 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) |
| Synonyms | PGP9.5, UCHL1, UCHL1/PGP9.5, PGP9/5, PGP 9.5 |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC000332 |
| Gene Symbol | UCHL1 |
| Gene ID (NCBI) | 7345 |
| RRID | AB_2919814 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09936 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 UCH-L1/PGP9.5 antibody CL594-14730 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
