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验证数据展示

  • WB analysis of HepG2 using 66191-1-Ig (same clone as 66191-1-PBS)

    WB analysis of HepG2 using 66191-1-Ig (same clone as 66191-1-PBS)

    WB result of VAPB antibody (66191-1-Ig, 1:5000) with si-control and si-VAPB transfected HepG2 cells. This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

  • WB analysis using 66191-1-Ig (same clone as 66191-1-PBS)

    WB analysis using 66191-1-Ig (same clone as 66191-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66191-1-Ig (VAPB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66191-1-Ig (same clone as 66191-1-PBS)

    IF Staining of HepG2 using 66191-1-Ig (same clone as 66191-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPB antibody (66191-1-Ig, Clone: 4F6A6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66191-1-Ig (same clone as 66191-1-PBS)

    IHC staining of human breast cancer using 66191-1-Ig (same clone as 66191-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66191-1-Ig (VAPB Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VAPB antibody included
  • IHC staining of human breast cancer using 66191-1-Ig (same clone as 66191-1-PBS)

    IHC staining of human breast cancer using 66191-1-Ig (same clone as 66191-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66191-1-Ig (VAPB Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66191-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VAPB antibody included
  • Featured Product
  • KD/KO Validated

VAPB Monoclonal antibody, PBS Only

VAPB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66191-1-PBS

Print datasheet

CloneNo. 4F6A6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ALS8, VAMP associated protein B/C, VAMP B, VAMP B/VAMP C, VAMP C, VAP B, VAP B/VAP C, VAP C, VAPB

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy VAPB Ready-To-Use IHC Kit. VAPB primary antibody included.

产品信息

66191-1-PBS targets VAPB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen VAPB fusion protein Ag6313 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name VAMP (vesicle-associated membrane protein)-associated protein B and C
Synonyms ALS8, VAMP associated protein B/C, VAMP B, VAMP B/VAMP C, VAMP C, VAP B, VAP B/VAP C, VAP C, VAPB
Calculated Molecular Weight 27 kDa
Observed Molecular Weight30-32 kDa
GenBank Accession NumberBC001712
Gene Symbol VAPB
Gene ID (NCBI) 9217
RRIDAB_2881586
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95292
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.