验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, LNCaP cells, MCF-7 cells, HeLa cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy VAPB Ready-To-Use IHC Kit. VAPB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66191-1-Ig targets VAPB in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, monkey, yeast |
| 免疫原 | VAPB fusion protein Ag6313 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | VAMP (vesicle-associated membrane protein)-associated protein B and C |
| 别名 | ALS8, VAMP associated protein B/C, VAMP B, VAMP B/VAMP C, VAMP C, VAP B, VAP B/VAP C, VAP C, VAPB |
| 计算分子量 | 27 kDa |
| 观测分子量 | 30-32 kDa |
| GenBank蛋白编号 | BC001712 |
| 基因名称 | VAPB |
| Gene ID (NCBI) | 9217 |
| RRID | AB_2881586 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O95292 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VAPB antibody 66191-1-Ig | Download protocol |
| IHC protocol for VAPB antibody 66191-1-Ig | Download protocol |
| IF protocol for VAPB antibody 66191-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Curr Biol The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.
| ||
J Cell Biol Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy. | ||
EMBO Rep Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling
| ||
J Biol Chem Proteomic mapping by rapamycin-dependent targeting of APEX2 identifies binding partners of VAPB at the inner nuclear membrane. | ||
