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CoraLite®594-conjugated VAPB Monoclonal antibody
VAPB Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
4F6A6
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy VAPB Ready-To-Use IHC Kit. VAPB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66191-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66191 targets VAPB in IF/ICC applications and shows reactivity with human samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | VAPB fusion protein Ag6313 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | VAMP (vesicle-associated membrane protein)-associated protein B and C |
| Synonyms | ALS8, VAMP associated protein B/C, VAMP B, VAMP B/VAMP C, VAMP C, VAP B, VAP B/VAP C, VAP C, VAPB |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC001712 |
| Gene Symbol | VAPB |
| Gene ID (NCBI) | 9217 |
| RRID | AB_2919962 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O95292 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 VAPB antibody CL594-66191 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |