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验证数据展示

  • FC experiment of Jurkat using CL555-14477

    FC experiment of Jurkat using CL555-14477

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 555 Anti-Human VAPB (CL555-14477) (red), or 0.2 ug Rabbit IgG CL555-30000(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HepG2 using CL555-14477

    FC experiment of HepG2 using CL555-14477

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 555-conjugated VAPB Polyclonal antibody (CL555-14477)(red), or 0.4 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 555-conjugated VAPB Polyclonal antibody

VAPB Polyclonal Antibody for FC (Intra)
Cat No. CL555-14477

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

VAMP C, VAMP B/VAMP C, VAMP B, VAMP associated protein B/C, UNQ484/PRO983

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 555
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inJurkat cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VAPB Ready-To-Use IHC Kit. VAPB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14477-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-14477 targets VAPB in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 VAPB fusion protein Ag5857 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 VAMP (vesicle-associated membrane protein)-associated protein B and C
别名 VAMP C, VAMP B/VAMP C, VAMP B, VAMP associated protein B/C, UNQ484/PRO983
计算分子量 27 kDa
观测分子量 27 kDa
GenBank蛋白编号BC001712
基因名称 VAPB
Gene ID (NCBI) 9217
RRIDAB_3084528
偶联类型 CoraLite® Plus 555 Fluorescent Dye
最大激发/发射波长554 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95292
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 VAPB antibody CL555-14477Download protocol
Standard Protocols
Click here to view our Standard Protocols