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验证数据展示

  • FC experiment of Jurkat using CL555-14477

    FC experiment of Jurkat using CL555-14477

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 555 Anti-Human VAPB (CL555-14477) (red), or 0.2 ug Rabbit IgG CL555-30000(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HepG2 using CL555-14477

    FC experiment of HepG2 using CL555-14477

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 555-conjugated VAPB Polyclonal antibody (CL555-14477)(red), or 0.4 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 555-conjugated VAPB Polyclonal antibody

VAPB Polyclonal Antibody for FC (Intra)
Cat No. CL555-14477

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

VAMP C, VAMP B/VAMP C, VAMP B, VAMP associated protein B/C, UNQ484/PRO983

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 555
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inJurkat cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VAPB Ready-To-Use IHC Kit. VAPB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14477-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-14477 targets VAPB in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen VAPB fusion protein Ag5857 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name VAMP (vesicle-associated membrane protein)-associated protein B and C
Synonyms VAMP C, VAMP B/VAMP C, VAMP B, VAMP associated protein B/C, UNQ484/PRO983
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC001712
Gene Symbol VAPB
Gene ID (NCBI) 9217
RRIDAB_3084528
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95292
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 VAPB antibody CL555-14477Download protocol
Standard Protocols
Click here to view our Standard Protocols