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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated VCP Monoclonal antibody

VCP Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A4B10

Cat no : CL488-60316

Print datasheet

Synonyms

15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP

验证数据展示

  • IF Staining of HeLa using CL488-60316

    IF Staining of HeLa using CL488-60316

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-60316 (VCP antibody) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy VCP Ready-To-Use IHC Kit. VCP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60316-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60316 targets VCP in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen VCP fusion protein Ag1002 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name valosin-containing protein
Synonyms 15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP
Calculated Molecular Weight 89 kDa
Observed Molecular Weight 89 kDa
GenBank Accession NumberBC007562
Gene Symbol VCP
Gene ID (NCBI) 7415
RRIDAB_2883141
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths488 nm / 515 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP55072
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 VCP antibody CL488-60316Download protocol
Standard Protocols
Click here to view our Standard Protocols