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Conjugate
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Conjugate
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验证数据展示

  • FC experiment of SH-SY5Y using CL488-55491

    FC experiment of SH-SY5Y using CL488-55491

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human VGLUT1 (CL488-55491) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse brain using CL488-55491

    IF Staining of mouse brain using CL488-55491

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 VGLUT1 antibody (CL488-55491) at dilution of 1:200.

CoraLite® Plus 488-conjugated VGLUT1 Polyclonal antibody

VGLUT1 Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-55491

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P, FC (Intra)

别名

SLC17A7, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, BNPI

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 55491-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-55491 targets VGLUT1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
别名 SLC17A7, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, BNPI
计算分子量 62 kDa
GenBank蛋白编号NM_020309
基因名称 VGLUT1
Gene ID (NCBI) 57030
RRIDAB_3084131
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P2U7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 VGLUT1 antibody CL488-55491Download protocol
Standard Protocols
Click here to view our Standard Protocols