验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy® VGLUT1 Ready-To-Use IHC Kit. VGLUT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 55491-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-55491 targets VGLUT1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF-P, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 |
| 别名 | 1, SLC17A7, BNPI, Brain-specific Na(+)-dependent inorganic phosphate cotransporter |
| 计算分子量 | 62 kDa |
| GenBank蛋白编号 | NM_020309 |
| 基因名称 | VGLUT1 |
| Gene ID (NCBI) | 57030 |
| RRID | AB_3084131 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9P2U7 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vesicular glutamate transporter 1 (VGLUT1) is a critical protein involved in the packaging and release of the neurotransmitter glutamate in synaptic vesicles. VGLUT1 is predominantly expressed in neurons that release glutamate, the primary excitatory neurotransmitter in the central nervous system, it is a marker of glutamatergic neurons.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 VGLUT1 antibody CL488-55491 | Download protocol |
| IF protocol for CL Plus 488 VGLUT1 antibody CL488-55491 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
