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CoraLite® Plus 488-conjugated VGLUT1 Polyclonal antibody

VGLUT1 Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-55491

Print datasheet

Synonyms

SLC17A7, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, BNPI

验证数据展示

  • FC experiment of SH-SY5Y using CL488-55491

    FC experiment of SH-SY5Y using CL488-55491

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human VGLUT1 (CL488-55491) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse brain using CL488-55491

    IF Staining of mouse brain using CL488-55491

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 VGLUT1 antibody (CL488-55491) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 55491-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-55491 targets VGLUT1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
Synonyms SLC17A7, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, BNPI
Calculated Molecular Weight 62 kDa
GenBank Accession NumberNM_020309
Gene Symbol VGLUT1
Gene ID (NCBI) 57030
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2U7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 VGLUT1 antibody CL488-55491Download protocol
Standard Protocols
Click here to view our Standard Protocols