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验证数据展示

  • IF Staining of human tonsillitis using CL488-60271

    IF Staining of human tonsillitis using CL488-60271

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-60271 (CD20 antibody) at dilution of 1:100.

  • IF Staining of human tonsillitis using CL488-60271

    IF Staining of human tonsillitis using CL488-60271

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-60271 (CD20 antibody) at dilution of 1:100.

CoraLite® Plus 488-conjugated CD20 Monoclonal antibody

CD20 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-60271

产品说明书

CloneNo. 4A7G3

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, IF-P

别名

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue, human lung cancer tissue, Raji cells
Positive IF/ICC detected inhuman tonsillitis tissue, human lung cancer tissue, Raji cells
Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60271-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60271 targets CD20 in IF/ICC, IF-P applications and shows reactivity with human samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 membrane-spanning 4-domains, subfamily A, member 1
别名 B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7
计算分子量 297 aa, 33 kDa
GenBank蛋白编号BC002807
基因名称 CD20
Gene ID (NCBI) 931
RRIDAB_2883132
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP11836
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells. This antibody is conjugated with CL488(Ex/Em 488 nm/515 nm).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD20 antibody CL488-60271Download protocol
Standard Protocols
Click here to view our Standard Protocols