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  • Featured Product
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ZAP70 Polyclonal antibody

ZAP70 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15592-1-AP

Print datasheet

Synonyms

Syk-related tyrosine kinase, STD, SRK, EC:2.7.10.2, 70 kDa zeta-chain associated protein

验证数据展示

  • WB analysis of Jurkat using 15592-1-AP

    WB analysis of Jurkat using 15592-1-AP

    WB result of ZAP70 antibody (15592-1-AP, 1:20000) with si-control and si-ZAP70 transfected Jurkat cells.

  • WB analysis using 15592-1-AP

    WB analysis using 15592-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 15592-1-AP

    IHC staining of human tonsillitis using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human tonsillitis using 15592-1-AP

    IHC staining of human tonsillitis using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of Jurkat using 15592-1-AP

    IP experiment of Jurkat using 15592-1-AP

    IP result of anti-ZAP70 (IP:15592-1-AP, 4ug; Detection:15592-1-AP 1:1000) with Jurkat cells lysate 3200ug.

  • IF Staining of RAW 264.7 using 15592-1-AP

    IF Staining of RAW 264.7 using 15592-1-AP

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using ZAP70 antibody (15592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, A375 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue, human kidney tissue, human lymphoma tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15592-1-AP targets ZAP70 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ZAP70 fusion protein Ag7943 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zeta-chain (TCR) associated protein kinase 70kDa
Synonyms Syk-related tyrosine kinase, STD, SRK, EC:2.7.10.2, 70 kDa zeta-chain associated protein
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC039039
Gene Symbol ZAP70
Gene ID (NCBI) 7535
ENSEMBL Gene IDENSG00000115085
RRIDAB_2218654
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43403
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

实验方案

Product Specific Protocols
WB protocol for ZAP70 antibody 15592-1-APDownload protocol
IHC protocol for ZAP70 antibody 15592-1-APDownload protocol
IF protocol for ZAP70 antibody 15592-1-APDownload protocol
IP protocol for ZAP70 antibody 15592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Immunol Methods

An optimized non-T cell transfection system based on HEK293FT cells for CD3ζ phosphorylation and ubiquitination

Authors - Jiaqi Zheng
View Article