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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ZAP70 Polyclonal antibody

ZAP70 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-15592

Print datasheet

Synonyms

70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

验证数据展示

  • IF Staining of RAW 264.7 using CL488-15592

    IF Staining of RAW 264.7 using CL488-15592

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CoraLite® Plus 488 ZAP70 antibody (CL488-15592) at dilution of 1:200.

经过测试的应用

Positive IF detected inRAW 264.7 cells
For other applications, we recommend the unconjugated version of this antibody, 15592-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15592 targets ZAP70 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen ZAP70 fusion protein Ag7943 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zeta-chain (TCR) associated protein kinase 70kDa
Synonyms 70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC039039
Gene Symbol ZAP70
Gene ID (NCBI) 7535
ENSEMBL Gene IDENSG00000115085
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43403
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ZAP70 antibody CL488-15592Download protocol
FC protocol for CL Plus 488 ZAP70 antibody CL488-15592Download protocol
Standard Protocols
Click here to view our Standard Protocols