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验证数据展示

  • FC experiment of Jurkat using CL488-15592

    FC experiment of Jurkat using CL488-15592

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Zap70 Polyclonal Antibody (CL488-15592)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of RAW 264.7 using CL488-15592

    IF Staining of RAW 264.7 using CL488-15592

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CoraLite® Plus 488 ZAP70 antibody (CL488-15592) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated ZAP70 Polyclonal antibody

ZAP70 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-15592

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Syk-related tyrosine kinase, STD, SRK, EC:2.7.10.2, 70 kDa zeta-chain associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inRAW 264.7 cells
Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 15592-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15592 targets ZAP70 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 ZAP70 fusion protein Ag7943 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zeta-chain (TCR) associated protein kinase 70kDa
别名 Syk-related tyrosine kinase, STD, SRK, EC:2.7.10.2, 70 kDa zeta-chain associated protein
计算分子量 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC039039
基因名称 ZAP70
Gene ID (NCBI) 7535
ENSEMBL Gene IDENSG00000115085
RRIDAB_3672634
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43403
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ZAP70 antibody CL488-15592Download protocol
FC protocol for CL Plus 488 ZAP70 antibody CL488-15592Download protocol
Standard Protocols
Click here to view our Standard Protocols