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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
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0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67447-1-Ig (same clone as 67447-1-PBS)

    WB analysis using 67447-1-Ig (same clone as 67447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67447-1-Ig (MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • WB analysis using 67447-1-Ig (same clone as 67447-1-PBS)

    WB analysis using 67447-1-Ig (same clone as 67447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67447-1-Ig (c-MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67447-1-Ig (same clone as 67447-1-PBS)

    IHC staining of human colon cancer using 67447-1-Ig (same clone as 67447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 67447-1-Ig (same clone as 67447-1-PBS)

    FC experiment of K-562 using 67447-1-Ig (same clone as 67447-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human c-MYC (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • FC experiment of NCCIT using 67447-1-Ig (same clone as 67447-1-PBS)

    FC experiment of NCCIT using 67447-1-Ig (same clone as 67447-1-PBS)

    1x10^6 NCCIT cells were intracellularly stained with 0.25 ug c-MYC Monoclonal antibody (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67447-1-Ig (same clone as 67447-1-PBS)

    FC experiment of HeLa using 67447-1-Ig (same clone as 67447-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug c-MYC Monoclonal antibody (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

c-MYC Monoclonal antibody, PBS Only

c-MYC Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 67447-1-PBS

产品说明书

CloneNo. 3D9C12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

MYC, MRTL, Class E basic helix-loop-helix protein 39, c Myc, bHLHe39

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

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产品信息

67447-1-PBS targets c-MYC in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 c-MYC fusion protein Ag17519 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 v-myc myelocytomatosis viral oncogene homolog (avian)
别名 MYC, MRTL, Class E basic helix-loop-helix protein 39, c Myc, bHLHe39
计算分子量 49 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC000141
基因名称 MYC
Gene ID (NCBI) 4609
RRIDAB_2882681
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01106
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.