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验证数据展示

  • FC experiment of HeLa using CL594-67447

    FC experiment of HeLa using CL594-67447

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human c-MYC (CL594-67447, Clone:3D9C12) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  • KD/KO验证

CoraLite®594-conjugated c-MYC Monoclonal antibody

c-MYC Monoclonal Antibody for FC (Intra)
Cat No. CL594-67447

产品说明书

CloneNo. 3D9C12

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

FC (Intra)

别名

MYC, Myc proto oncogene protein, Myc proto-oncogene protein, Proto-oncogene c-Myc, 3D9C12, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39, c-Myc, MRTL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67447-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67447 targets c-MYC in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, mouse, rat
免疫原 c-MYC fusion protein Ag17519 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 v-myc myelocytomatosis viral oncogene homolog (avian)
别名 MYC, Myc proto oncogene protein, Myc proto-oncogene protein, Proto-oncogene c-Myc, 3D9C12, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39, c-Myc, MRTL
计算分子量 49 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC000141
基因名称 MYC
Gene ID (NCBI) 4609
RRIDAB_3084767
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP01106
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.

实验方案

Product Specific Protocols
FC protocol for CL594 c-MYC antibody CL594-67447Download protocol
Standard Protocols
Click here to view our Standard Protocols