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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated c-MYC Monoclonal antibody

c-MYC Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D9C12

Cat no : CL594-67447

Print datasheet

Synonyms

MYC, Myc proto oncogene protein, Myc proto-oncogene protein, Proto-oncogene c-Myc, 3D9C12, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39, c-Myc, MRTL

验证数据展示

  • FC experiment of HeLa using CL594-67447

    FC experiment of HeLa using CL594-67447

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human c-MYC (CL594-67447, Clone:3D9C12) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67447-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67447 targets c-MYC in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, mouse, rat
Immunogen c-MYC fusion protein Ag17519 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Synonyms MYC, Myc proto oncogene protein, Myc proto-oncogene protein, Proto-oncogene c-Myc, 3D9C12, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39, c-Myc, MRTL
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC000141
Gene Symbol MYC
Gene ID (NCBI) 4609
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP01106
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.

实验方案

Product Specific Protocols
FC protocol for CL594 c-MYC antibody CL594-67447Download protocol
Standard Protocols
Click here to view our Standard Protocols