- Featured Product
- KD/KO Validated
CoraLite® Plus 555-conjugated calreticulin Polyclonal antibody
calreticulin Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra) and More (1)
Conjugate
CoraLite® Plus 555 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells, SKOV-3 cells |
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CALR Ready-To-Use IHC Kit. CALR primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 27298-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL555-27298 targets calreticulin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | calreticulin fusion protein Ag26064 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | calreticulin |
| Synonyms | CALR, Calregulin, calreticulin, cC1qR, CRP55, CRT, CRTC, ERp60, FLJ26680, grp60, HACBP, RO, SSA |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC002500 |
| Gene Symbol | Calreticulin |
| Gene ID (NCBI) | 811 |
| Conjugate | CoraLite® Plus 555 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 554 nm / 570 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27797 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CALR, also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is an ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 60 kD by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 555 calreticulin antibody CL555-27298 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
