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CoraLite®594-conjugated Gamma Tubulin Monoclonal antibody

Gamma Tubulin Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, canine

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3F9H8

Cat no : CL594-66320

Print datasheet

Synonyms

TUBG1, TUBG, GCP-1, Gamma-tubulin complex component 1, Gamma-1-tubulin

验证数据展示

  • IF Staining of MDCK using CL594-66320

    IF Staining of MDCK using CL594-66320

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using CL594-66320 (gamma tubulin antibody) at dilution of 1:50.

  • FC experiment of HeLa using CL594-66320

    FC experiment of HeLa using CL594-66320

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Gamma Tubulin (CL594-66320, Clone:3F9H8) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inMDCK cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66320-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66320 targets Gamma Tubulin in IF/ICC, FC (Intra) applications and shows reactivity with human, canine samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, canine
Immunogen Gamma Tubulin fusion protein Ag23913 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name tubulin, gamma 1
Synonyms TUBG1, TUBG, GCP-1, Gamma-tubulin complex component 1, Gamma-1-tubulin
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 48-55 kDa
GenBank Accession NumberBC000619
Gene Symbol Gamma Tubulin
Gene ID (NCBI) 7283
RRIDAB_2883556
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23258
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.

实验方案

Product Specific Protocols
IF protocol for CL594 Gamma Tubulin antibody CL594-66320Download protocol
FC protocol for CL594 Gamma Tubulin antibody CL594-66320Download protocol
Standard Protocols
Click here to view our Standard Protocols