验证数据展示
经过测试的应用
Positive IF/ICC detected in | MDCK cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66320 targets Gamma Tubulin in IF/ICC, FC (Intra) applications and shows reactivity with human, canine samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human, canine |
Immunogen | Gamma Tubulin fusion protein Ag23913 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | tubulin, gamma 1 |
Synonyms | TUBG1, TUBG, GCP-1, Gamma-tubulin complex component 1, Gamma-1-tubulin |
Calculated Molecular Weight | 51 kDa |
Observed Molecular Weight | 48-55 kDa |
GenBank Accession Number | BC000619 |
Gene Symbol | Gamma Tubulin |
Gene ID (NCBI) | 7283 |
RRID | AB_2883556 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P23258 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 Gamma Tubulin antibody CL594-66320 | Download protocol |
FC protocol for CL594 Gamma Tubulin antibody CL594-66320 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |