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验证数据展示

  • IF Staining of MDCK using CL594-66320

    IF Staining of MDCK using CL594-66320

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using CL594-66320 (gamma tubulin antibody) at dilution of 1:50.

  • FC experiment of HeLa using CL594-66320

    FC experiment of HeLa using CL594-66320

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Gamma Tubulin (CL594-66320, Clone:3F9H8) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated Gamma Tubulin Monoclonal antibody

Gamma Tubulin Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66320

产品说明书

CloneNo. 3F9H8

宿主/亚型

Mouse / IgG2a

种属反应性

human, canine

应用

IF/ICC, FC (Intra)

别名

TUBG1, TUBG, GCP-1, Gamma-tubulin complex component 1, Gamma-1-tubulin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMDCK cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66320-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66320 targets Gamma Tubulin in IF/ICC, FC (Intra) applications and shows reactivity with human, canine samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, canine
免疫原 Gamma Tubulin fusion protein Ag23913 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 tubulin, gamma 1
别名 TUBG1, TUBG, GCP-1, Gamma-tubulin complex component 1, Gamma-1-tubulin
计算分子量 51 kDa
观测分子量 48-55 kDa
GenBank蛋白编号BC000619
基因名称 Gamma Tubulin
Gene ID (NCBI) 7283
RRIDAB_2883556
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23258
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.

实验方案

Product Specific Protocols
IF protocol for CL594 Gamma Tubulin antibody CL594-66320Download protocol
FC protocol for CL594 Gamma Tubulin antibody CL594-66320Download protocol
Standard Protocols
Click here to view our Standard Protocols