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Validation Data Gallery

  • Purity of recombinant human Activin A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human Activin A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human Activin A (HZ-1138) causes dose-dependent inhibition of proliferation in the Mouse plasmacytoma (MPC-11) cell line. Proliferation of the MPC-11 cell line was assessed using Promega CellTiter 96®. MPC-11 cells were treated with increasing concentrations of recombinant human Activin A for 72 hrs hours before addition of CellTiter96® reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.5-3.5 ng/ml.

    Recombinant human Activin A (HZ-1138) causes dose-dependent inhibition of proliferation in the Mouse plasmacytoma (MPC-11) cell line. Proliferation of the MPC-11 cell line was assessed using Promega CellTiter 96®. MPC-11 cells were treated with increasing concentrations of recombinant human Activin A for 72 hrs hours before addition of CellTiter96® reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.5-3.5 ng/ml.

  • iPSC-derived Cardiomyocytes Cultured using Recombinant Human Activin A, BMP-4 and bFGF/FGF-2).​ iPSCs differentiated to Cardiomyocytes using HumanKine® recombinant human Activin A protein (HZ-1138), HumanKine® recombinant human BMP-4 protein (HZ-1045), HumanKine® recombinant human bFGF/FGF-2 (HZ-1285), were fixed and permeabilized for detection of endogenous Alpha actinin and Cardiac Troponin T using Alpha Actinin Polyclonal Antibody (Product # 11313-2-AP, 1:200 dilution, labelled with CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4)) and Cardiac Troponin T Polyclonal Antibody (Product # 15513-1-AP, 1:200 dilution, labelled with CoraLite®488- Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)).

    iPSC-derived Cardiomyocytes Cultured using Recombinant Human Activin A, BMP-4 and bFGF/FGF-2).​ iPSCs differentiated to Cardiomyocytes using HumanKine® recombinant human Activin A protein (HZ-1138), HumanKine® recombinant human BMP-4 protein (HZ-1045), HumanKine® recombinant human bFGF/FGF-2 (HZ-1285), were fixed and permeabilized for detection of endogenous Alpha actinin and Cardiac Troponin T using Alpha Actinin Polyclonal Antibody (Product # 11313-2-AP, 1:200 dilution, labelled with CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4)) and Cardiac Troponin T Polyclonal Antibody (Product # 15513-1-AP, 1:200 dilution, labelled with CoraLite®488- Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)).

  • Human induced pluripotent cells(iPSCs) were differentiated to definitive endoderm using HumanKine growth factors (Activin A- HZ-1138, bFGF-HZ-1285, BMP-4- HZ-1045, and Wnt3A-HZ-1296). The differentiation was confirmed by detection of expression of FOXA2(Red) and downregulation of pluripotency marker NANOG(yellow), in immunofluorescence

    Human induced pluripotent cells(iPSCs) were differentiated to definitive endoderm using HumanKine growth factors (Activin A- HZ-1138, bFGF-HZ-1285, BMP-4- HZ-1045, and Wnt3A-HZ-1296). The differentiation was confirmed by detection of expression of FOXA2(Red) and downregulation of pluripotency marker NANOG(yellow), in immunofluorescence

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human Activin A protein

Cat No. HZ-1138
引用文献(37)

Activity

0.5-3.5 ng/mL

Specific Activity

minimally 4.00 x 102 IU/mg

Species Reactivity

human,mouse

Purity

>95%

Synonyms

Activin A, Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, inhibin, beta A

Product type:  Research use only
规格价格库存

GMP-Grade HumanKine® Info

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Technical Specifications

GeneID 3624
Species Human
Expression HEK293
Activity 0.5-3.5 ng/mL
Specific Activityminimally 4.00 x 102 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP08476
Molecular Mass 13kDa reduced, 22 kDa non-reduced, homodimer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently Swirl or tap vial to mix

Background

HumanKine Activin A is a high-quality, animal-free recombinant human protein widely used in stem cell research, cell culture, and signaling pathway studies. Produced in HEK293 cells, it is designed to deliver exceptional purity, strong bioactivity, and low endotoxin levels for consistent experimental performance. HumanKine Activin A supports applications such as stem cell differentiation, tissue development, regenerative medicine research, and advanced biological assay development. Activin A is a dimeric growth factor within the TGF-β superfamily that engages specific type II and type I receptors to initiate SMAD2/3 signaling cascades and downstream transcriptional regulation. Functionally, Activin A is a key regulator of pluripotent stem cell maintenance and a potent inducer of definitive endoderm formation Its reliable human-like glycosylation profile makes it a preferred choice for researchers seeking reproducible results in sensitive cell-based workflows.(PMID 16885530, 8200957)

Synonyms

Activin A, Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, inhibin, beta A

Publications

SpeciesTitle
human

Cell Res

Visualization of aging-associated chromatin alterations with an engineered TALE system.

Authors - Ruotong Ren
View Article
human

Nat Protoc

The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor-like cells.

Authors - Yun Xia
View Article
human

Protein Cell

CRISPR/Cas9-mediated gene knockout reveals a guardian role of NF-κB/RelA in maintaining the homeostasis of human vascular cells.

Authors - Ping Wang
View Article
human

Protein Cell

Modeling CADASIL vascular pathologies with patient-derived induced pluripotent stem cells.

Authors - Chen Ling
View Article
human

Nat Commun

Selectively targeting the AdipoR2-CaM-CaMKII-NOS3 axis by SCM-198 as a rapid-acting therapy for advanced acute liver failure

Authors - Rui Wang
View Article
human

Nat Commun

Live imaging and functional characterization of the avian hypoblast redefine the mechanisms of primitive streak induction.

Authors - Aurélien Villedieu
View Article
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