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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human Activin A protein

Activity

0.5-3.5 ng/mL

Specific Activity

minimally 4.00 x 102 IU/mg

Species Reactivity

human,mouse

Purity

>95%

Cat No : HZ-1138

Print datasheet

Synonyms

Activin A, Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, inhibin, beta A

Validation Data Gallery

  • Purity of recombinant human Activin A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human Activin A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human Activin A (HZ-1138) causes dose-dependent inhibition of proliferation in the Mouse plasmacytoma (MPC-11) cell line. Proliferation of the MPC-11 cell line was assessed using Promega CellTiter 96®. MPC-11 cells were treated with increasing concentrations of recombinant human Activin A for 72 hrs hours before addition of CellTiter96® reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.5-3.5 ng/ml.

    Recombinant human Activin A (HZ-1138) causes dose-dependent inhibition of proliferation in the Mouse plasmacytoma (MPC-11) cell line. Proliferation of the MPC-11 cell line was assessed using Promega CellTiter 96®. MPC-11 cells were treated with increasing concentrations of recombinant human Activin A for 72 hrs hours before addition of CellTiter96® reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.5-3.5 ng/ml.

  • Human induced pluripotent cells(iPSCs) were differentiated to definitive endoderm using HumanKine growth factors (Activin A- HZ-1138, bFGF-HZ-1285, BMP-4- HZ-1045, and Wnt3A-HZ-1296). The differentiation was confirmed by detection of expression of FOXA2(Red) and downregulation of pluripotency marker NANOG(yellow), in immunofluorescence

    Human induced pluripotent cells(iPSCs) were differentiated to definitive endoderm using HumanKine growth factors (Activin A- HZ-1138, bFGF-HZ-1285, BMP-4- HZ-1045, and Wnt3A-HZ-1296). The differentiation was confirmed by detection of expression of FOXA2(Red) and downregulation of pluripotency marker NANOG(yellow), in immunofluorescence

Technical Specifications

GeneID 3624
Species Human
Expression HEK293
Activity 0.5-3.5 ng/mL
Specific Activityminimally 4.00 x 102 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP08476
Molecular Mass 13kDa reduced, 22 kDa non-reduced, homodimer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently Swirl or tap vial to mix

Background

Activin A, a member of the TGF-β superfamily, was named for its ability to activate FSH secretion from the anterior pituitary gland (PMID 16885530). Three activins have been identified in human tissues: activin A, activin B, and activinAB, of which activin A is the most studied. Activin A has various functions in regulating cell differentiation, proliferation, apoptosis and carcinogenesis (PMID 8200957). Xeno-free Recombinant Human Activin A is expressed from human 293 cells as disulfide linked homodimers with molecular mass of 25 kDa.

Synonyms

Activin A, Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, inhibin, beta A

Publications

SpeciesTitle
human

Cell Res

Visualization of aging-associated chromatin alterations with an engineered TALE system.

Authors - Ruotong Ren
View Article
human

Biomater Res

Self-organized insulin-producing β-cells differentiated from human omentum-derived stem cells and their in vivo therapeutic potential

Authors - Ji Hoon Jeong
View Article
mouse

EMBO J

HIF1α induced switch from bivalent to exclusively glycolytic metabolism during ESC-to-EpiSC/hESC transition.

Authors - WenyuZhou
View Article
human,mouse

Proc Natl Acad Sci U S A

Derivation of naive human embryonic stem cells.

Authors - Carol BWare
View Article
human

Nat Protoc

The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor-like cells.

Authors - Yun Xia
View Article
human

Protein Cell

CRISPR/Cas9-mediated gene knockout reveals a guardian role of NF-κB/RelA in maintaining the homeostasis of human vascular cells.

Authors - Ping Wang
View Article