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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human LIF protein

Activity

0.135-0.675 ng/mL TF-1 Cell assay. 0.045-0.25 ng/mL EC50 in M1 cell assay

Specific Activity

minimally 3.50 x 106 IU/mg

Species Reactivity

human,mouse

Purity

>95%

Cat No : HZ-1292

Print datasheet

Synonyms

CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI

Validation Data Gallery

  • Purity of recombinant human LIF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human LIF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human LIF (HZ-1292) dose-dependently inhibits growth of the M1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. M1 cells were treated with increasing concentrations of recombinant LIF for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.045-0.25 ng/mL​.

    Recombinant human LIF (HZ-1292) dose-dependently inhibits growth of the M1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. M1 cells were treated with increasing concentrations of recombinant LIF for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.045-0.25 ng/mL​.

  • The activity was determined by the dose­-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

    The activity was determined by the dose­-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

Technical Specifications

GeneID 3976
Species Human
Expression HEK293
Activity 0.135-0.675 ng/mL TF-1 Cell assay. 0.045-0.25 ng/mL EC50 in M1 cell assay
Specific Activityminimally 3.50 x 106 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP15018
Molecular Mass 35-55 kDa reduced and non-reduced, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

LIF is a pleiotropic cytokine with a variety of roles. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface (PMID: 29620145; 29391217; 29271384)

Synonyms

CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI

Publications

SpeciesTitle
mouse

Nucleic Acids Res

Chromatin-contact atlas reveals disorder-mediated protein interactions and moonlighting chromatin-associated RBPs.

Authors - Mahmoud-Reza Rafiee
View Article

Reprod Biol Endocrinol

Expression of transcriptional factor EB (TFEB) in differentiating spermatogonia potentially promotes cell migration in mouse seminiferous epithelium.

Authors - Yue Liu
View Article

Brain Commun

Human stem cell-derived ventral midbrain astrocytes exhibit a region-specific secretory profile

Authors - Lucy A Crompton
View Article
human

Biochem Pharmacol

Enhanced binding of β-catenin and β-TrCP mediates LMPt's anti-CSCs activity in colorectal cancer

Authors - Mengyan Wang
View Article