验证数据展示

IF Staining of rat heart using 67735-1-Ig
Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using a-SMA specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IHC staining of human colon using 67735-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67735-1-Ig (a-SMA specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with smooth muscle actin antibody included
WB analysis using 67735-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (a-SMA specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
FC experiment of C2C12 using 67735-1-Ig
1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human smooth muscle actin specific (67735-1-Ig, Clone:1E9A11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
WB analysis using 67735-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
IF Staining of mouse breast cancer using 67735-1-Ig
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using Alpha smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).
IF Staining of C2C12 using 67735-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Alpha smooth muscle actin specific Monoclonal antibody

Alpha smooth muscle actin specific Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

Cat No. 67735-1-Ig

产品说明书

CloneNo. 1E9A11

引用文献(149)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit and More (1)

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA and More (2)

别名

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, 1E9A11

缓冲液配方: PBS and Azide

PBS and Azide

PBS Only

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®594

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (149)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	rat stomach tissue, rabbit stomach tissue, pig vein tissue, pig stomach tissue, rabbit large intestine tissue, pig colon tissue, rat colon tissue, mouse colon tissue
Positive IHC detected in	human colon tissue, human appendicitis tissue, human liver tissue, human liver cancer tissue, rat colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	rat heart tissue
Positive IF-Fro detected in	mouse breast cancer
Positive IF/ICC detected in	C2C12 cells
Positive FC (Intra) detected in	C2C12 cells

Planning an IHC experiment? We recommend our IHCeasy smooth muscle actin Ready-To-Use IHC Kit. smooth muscle actin primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:20000-1:100000
Immunohistochemistry (IHC)	IHC : 1:2500-1:10000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)-FRO	IF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 69 publications below
IHC	See 36 publications below
IF	See 92 publications below
CoIP	See 1 publications below

产品信息

67735-1-Ig targets Alpha smooth muscle actin specific in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用	WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA Application Description
文献引用应用	WB, IHC, IF, CoIP
经测试反应性	human, mouse, rat, pig, rabbit
文献引用反应性	human, mouse, rat, pig, zebrafish
免疫原	Peptide 种属同源性预测
宿主/亚型	Mouse / IgG1
抗体类别	Monoclonal
产品类型	Antibody
全称	actin, alpha 2, smooth muscle, aorta
别名	ACTA2, Alpha SMA, a-SMA, smooth muscle actin, 1E9A11
计算分子量	42 kDa
观测分子量	42 kDa
GenBank蛋白编号	NM_001613
基因名称	Alpha smooth muscle actin
Gene ID (NCBI)	59
RRID	AB_2918504
偶联类型	Unconjugated
形式	Liquid
纯化方式	Protein G purification
UNIPROT ID	P62736
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

实验方案

Product Specific Protocols
WB protocol for Alpha smooth muscle actin specific antibody 67735-1-Ig	Download protocol
IHC protocol for Alpha smooth muscle actin specific antibody 67735-1-Ig	Download protocol
IF protocol for Alpha smooth muscle actin specific antibody 67735-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IF	Adv Mater A "Bridge-Building" Glycan Scaffold Mimicking Microbial Invasion for In Situ Endothelialization. Authors - Ruoyu Mu View Article
mouse	IF	Nat Commun ALKBH5-mediated m6A modification of IL-11 drives macrophage-to-myofibroblast transition and pathological cardiac fibrosis in mice Authors - Tao Zhuang View Article
human	IHC	Mol Cancer circFARP1 enables cancer-associated fibroblasts to promote gemcitabine resistance in pancreatic cancer via the LIF/STAT3 axis. Authors - Chonghui Hu View Article
human	IF	Adv Sci (Weinh) Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma Authors - Yongxu Jia View Article
mouse	IF	Acta Pharm Sin B ADAR1 regulates vascular remodeling in hypoxic pulmonary hypertension through N1-methyladenosine modification of circCDK17 Authors - Junting Zhang View Article
rat	WB	Biomaterials Extended-release of therapeutic microRNA via a host-guest supramolecular hydrogel to locally alleviate renal interstitial fibrosis. Authors - Yingying Xu View Article

验证数据展示

IF Staining of rat heart using 67735-1-Ig

IHC staining of human colon using 67735-1-Ig

WB analysis using 67735-1-Ig

FC experiment of C2C12 using 67735-1-Ig

WB analysis using 67735-1-Ig

IF Staining of mouse breast cancer using 67735-1-Ig

IF Staining of C2C12 using 67735-1-Ig

Alpha smooth muscle actin specific Monoclonal antibody

Alpha smooth muscle actin specific Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

A "Bridge-Building" Glycan Scaffold Mimicking Microbial Invasion for In Situ Endothelialization.

ALKBH5-mediated m6A modification of IL-11 drives macrophage-to-myofibroblast transition and pathological cardiac fibrosis in mice

circFARP1 enables cancer-associated fibroblasts to promote gemcitabine resistance in pancreatic cancer via the LIF/STAT3 axis.

Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma

ADAR1 regulates vascular remodeling in hypoxic pulmonary hypertension through N1-methyladenosine modification of circCDK17

Extended-release of therapeutic microRNA via a host-guest supramolecular hydrogel to locally alleviate renal interstitial fibrosis.