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smooth muscle actin specific Monoclonal antibody, PBS Only

smooth muscle actin specific Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rabbit, pig, rat, mouse, human

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E9A11

Cat no : 67735-1-PBS

Print datasheet

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, actin

验证数据展示

  • IF Staining of rat heart using 67735-1-Ig (same clone as 67735-1-PBS)

    IF Staining of rat heart using 67735-1-Ig (same clone as 67735-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using a-SMA specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 67735-1-Ig (same clone as 67735-1-PBS)

    IHC staining of human colon using 67735-1-Ig (same clone as 67735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67735-1-Ig (a-SMA specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with smooth muscle actin antibody included
  • WB analysis using 67735-1-Ig (same clone as 67735-1-PBS)

    WB analysis using 67735-1-Ig (same clone as 67735-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (a-SMA specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

  • FC experiment of C2C12 using 67735-1-Ig (same clone as 67735-1-PBS)

    FC experiment of C2C12 using 67735-1-Ig (same clone as 67735-1-PBS)

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human smooth muscle actin specific (67735-1-Ig, Clone:1E9A11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

  • WB analysis using 67735-1-Ig (same clone as 67735-1-PBS)

    WB analysis using 67735-1-Ig (same clone as 67735-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 67735-1-Ig (same clone as 67735-1-PBS)

    IF Staining of C2C12 using 67735-1-Ig (same clone as 67735-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy smooth muscle actin Ready-To-Use IHC Kit. smooth muscle actin primary antibody included.

产品信息

67735-1-PBS targets smooth muscle actin specific in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity rabbit, pig, rat, mouse, human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name actin, alpha 2, smooth muscle, aorta
Synonyms ACTA2, Alpha SMA, a-SMA, smooth muscle actin, actin
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberNM_001613
Gene Symbol Alpha smooth muscle actin
Gene ID (NCBI) 59
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62736
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.