验证数据展示
经过测试的应用
Positive IF-P detected in | human lung cancer tissue, human tonsillitis tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-60271 targets CD20 in IF-P applications and shows reactivity with human samples.
经测试应用 | IF-P Application Description |
经测试反应性 | human |
免疫原 | CD20 fusion protein Ag17309 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | membrane-spanning 4-domains, subfamily A, member 1 |
别名 | B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7 |
计算分子量 | 297 aa, 33 kDa |
GenBank蛋白编号 | BC002807 |
基因名称 | CD20 |
Gene ID (NCBI) | 931 |
RRID | AB_2883651 |
偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
最大激发/发射波长 | 654 nm / 674 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P11836 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 647 CD20 antibody CL647-60271 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |