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Glycophorin A Polyclonal antibody

Glycophorin A Polyclonal Antibody for FC, IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15874-1-AP

Print datasheet

Synonyms

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha

验证数据展示

  • WB analysis of K-562 using 15874-1-AP

    WB analysis of K-562 using 15874-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15874-1-AP (Glycophorin A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 15874-1-AP

    IHC staining of human spleen using 15874-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15874-1-AP (Glycophorin A antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • IHC staining of human spleen using 15874-1-AP

    IHC staining of human spleen using 15874-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15874-1-AP (Glycophorin A antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • IF Staining of human placenta using 15874-1-AP

    IF Staining of human placenta using 15874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (15874-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human placenta using 15874-1-AP

    IF Staining of human placenta using 15874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (15874-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of K-562 using 15874-1-AP

    FC experiment of K-562 using 15874-1-AP

    1x10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Glycophorin A (15874-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were not fixed.

经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman spleen tissue, human lung tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman placenta tissue
Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:300-1:1200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15874-1-AP targets Glycophorin A in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF-P, IHC, WB, ELISA
Tested Reactivity human
Immunogen Glycophorin A fusion protein Ag8635 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycophorin A (MNS blood group)
Synonyms CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha
Calculated Molecular Weight 150 aa, 16 kDa
Observed Molecular Weight 36-38 kDa
GenBank Accession NumberBC005319
Gene Symbol GYPA
Gene ID (NCBI) 2993
RRIDAB_2116233
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02724
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

实验方案

Product Specific Protocols
WB protocol for Glycophorin A antibody 15874-1-APDownload protocol
IHC protocol for Glycophorin A antibody 15874-1-APDownload protocol
IF protocol for Glycophorin A antibody 15874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols