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验证数据展示

  • IF Staining of human placenta using CL488-15874

    IF Staining of human placenta using CL488-15874

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite® Plus 488 Glycophorin A antibody (CL488-15874) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human placenta using CL488-15874

    IF Staining of human placenta using CL488-15874

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite® Plus 488 Glycophorin A antibody (CL488-15874) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated Glycophorin A/CD235a Polyclonal antibody

Glycophorin A/CD235a Polyclonal Antibody for IF-P
Cat No. CL488-15874

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF-P

别名

Glycophorin A, CD235a, GpMiIII, GPErik, GPA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15874-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15874 targets Glycophorin A/CD235a in IF-P applications and shows reactivity with human samples.

经测试应用 IF-P Application Description
经测试反应性 human
免疫原 Glycophorin A/CD235a fusion protein Ag8635 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycophorin A (MNS blood group)
别名 Glycophorin A, CD235a, GpMiIII, GPErik, GPA
计算分子量 150 aa, 16 kDa
观测分子量 36-38 kDa
GenBank蛋白编号BC005319
基因名称 Glycophorin A
Gene ID (NCBI) 2993
RRIDAB_3672641
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02724
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Glycophorin A/CD235a antibody CL488-15874Download protocol
Standard Protocols
Click here to view our Standard Protocols