Hemoglobin Epsilon Polyclonal antibody

Hemoglobin Epsilon Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 12361-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

HBE1, Hemoglobin epsilon-Specific, Hemoglobin epsilon chain, HBE, Epsilon-globin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman placenta tissue, K-562 cells, mouse placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inK-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12361-1-AP targets Hemoglobin Epsilon in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Hemoglobin Epsilon fusion protein Ag3026 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hemoglobin, epsilon 1
别名 HBE1, Hemoglobin epsilon-Specific, Hemoglobin epsilon chain, HBE, Epsilon-globin
计算分子量 147 aa, 16 kDa
观测分子量 12-16 kDa
GenBank蛋白编号BC015537
基因名称 HBE1
Gene ID (NCBI) 3046
RRIDAB_2114593
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02100
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBE1 (hemoglobin epsilon chain) is a beta-type chain of hemoglobin predominantly expressed during the embryonic stage (21321359). The HBE1 has been regarded as the best marker for fetal nucleated red blood cells (NRBCs) . This antibody can be used to label and isolate fetal cells from maternal blood and may be useful in prenatal diagnosis (15906407).

实验方案

Product Specific Protocols
WB protocol for Hemoglobin Epsilon antibody 12361-1-APDownload protocol
IHC protocol for Hemoglobin Epsilon antibody 12361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Blood

NFI-A directs the fate of hematopoietic progenitors to the erythroid or granulocytic lineage and controls beta-globin and G-CSF receptor expression.

Authors - Linda Marie Starnes
humanWB

Cell Death Differ

Transcriptional fine-tuning of microRNA-223 levels directs lineage choice of human hematopoietic progenitors.

Authors - L Vian
humanWB

Mol Cell Biol

A Feedback Loop Consisting of MicroRNA 23a/27a and the β-Like Globin Suppressors KLF3 and SP1 Regulates Globin Gene Expression.

Authors - Ma Yanni Y
humanWB

Biochim Biophys Acta Mol Cell Res

Long noncoding RNA CCDC26 as a modulator of transcriptional switching between fetal and embryonic globins.

Authors - Tetsuo Hirano
ratWB

Proteome Sci

A comparison of E15.5 fetus and newborn rat serum proteomes.

Authors - Wei Lilong L
mouseIHC

Blood

Requirement for antiapoptotic MCL-1 during early erythropoiesis

Authors - Meghan E Turnis
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