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Beta Tubulin Polyclonal antibody

Beta Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10068-1-AP

Print datasheet

Synonyms

Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain

验证数据展示

  • IF Staining of C2C12 using 10068-1-AP

    IF Staining of C2C12 using 10068-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Beta Tubulin antibody (10068-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IP experiment of HEK-293 using 10068-1-AP

    IP experiment of HEK-293 using 10068-1-AP

    IP result of anti-Beta Tubulin (IP:10068-1-AP, 4ug; Detection:10068-1-AP 1:5000) with HEK-293 cells lysate 2320 ug.

  • IHC staining of mouse brain using 10068-1-AP

    IHC staining of mouse brain using 10068-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10068-1-AP (beta Tubulin antibody at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IHC staining of mouse brain using 10068-1-AP

    IHC staining of mouse brain using 10068-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10068-1-AP (beta Tubulin antibody at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with TUBB3 antibody included
  • WB analysis using 10068-1-AP

    WB analysis using 10068-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10068-1-AP (beta Tubulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10068-1-AP

    WB analysis using 10068-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10068-1-AP (Beta Tubulin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 10068-1-AP

    FC experiment of HeLa using 10068-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Beta Tubulin (10068-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse cerebellum tissue, HeLa cells, mouse brain tissue, rat brain tissue, HEK-293 cells, Jurkat cells, HepG2 cells, A431 cells, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse brain tissue, human brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10068-1-AP targets Beta Tubulin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, zebrafish, bovine, silkworm, caenorhabditis elegans
Immunogen Beta Tubulin fusion protein Ag0117 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin, beta 3
Synonyms Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain
Calculated Molecular Weight 450 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC000748
Gene Symbol TUBB3
Gene ID (NCBI) 10381
RRIDAB_2303998
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13509
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

实验方案

Product Specific Protocols
WB protocol for Beta Tubulin antibody 10068-1-APDownload protocol
IHC protocol for Beta Tubulin antibody 10068-1-APDownload protocol
IF protocol for Beta Tubulin antibody 10068-1-APDownload protocol
IP protocol for Beta Tubulin antibody 10068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

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