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Cytokeratin 20 Recombinant antibody

Cytokeratin 20 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2H10

Cat no : 82428-1-RR

Print datasheet

Synonyms

KRT20, keratin 20, K20, Cytokeratin-20, CK-20

验证数据展示

  • WB analysis using 82428-1-RR

    WB analysis using 82428-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:22000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 82428-1-RR

    IHC staining of human colon cancer using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT20 antibody included
  • IHC staining of human colon cancer using 82428-1-RR

    IHC staining of human colon cancer using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT20 antibody included
  • IF Staining of HT-29 using 82428-1-RR

    IF Staining of HT-29 using 82428-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HT-29 using 82428-1-RR

    FC experiment of HT-29 using 82428-1-RR

    1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 20 (82428-1-RR, Clone:2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82428-1-RR

    Affinity and Kinetic Characterization of 82428-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82428-1-RR against Human Cytokeratin 20 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHT-29 cells, COLO 320 cells, pig colon tissue, mouse colon tissue, rat colon tissue
Positive IHC detected inhuman colon cancer tissue, human appendicitis tissue, human colon tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHT-29 cells
Positive FC (Intra) detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy KRT20 Ready-To-Use IHC Kit. KRT20 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82428-1-RR targets Cytokeratin 20 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Cytokeratin 20 fusion protein Ag11308 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name keratin 20
Synonyms KRT20, keratin 20, K20, Cytokeratin-20, CK-20
Calculated Molecular Weight 424 aa, 48 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC031559
Gene Symbol Cytokeratin 20
Gene ID (NCBI) 54474
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35900
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
IHC protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
IF protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols