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  • Featured Product
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ASGR1 Polyclonal antibody

ASGR1 Polyclonal Antibody for WB, IHC, IF-P, FC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, FC, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 11739-1-AP

Print datasheet

Synonyms

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

验证数据展示

  • WB analysis using 11739-1-AP

    WB analysis using 11739-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat liver using 11739-1-AP

    IHC staining of rat liver using 11739-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11739-1-AP (ASGR1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ASGR1 antibody included
  • IHC staining of rat liver using 11739-1-AP

    IHC staining of rat liver using 11739-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11739-1-AP (ASGR1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ASGR1 antibody included
  • IF Staining of human liver using 11739-1-AP

    IF Staining of human liver using 11739-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver tissue using ASGR1 antibody (11739-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA502, orange), CoraLite® Plus 488 CD163 antibody (CL488-16646, green), CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005, magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 11739-1-AP

    FC experiment of HepG2 using 11739-1-AP

    1x10^6 HepG2 cells were surface stained with 0.4 ug ASGR1 Polyclonal antibody (11739-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were not fixed.

  • IP experiment of mouse liver using 11739-1-AP

    IP experiment of mouse liver using 11739-1-AP

    IP result of anti-ASGR1 (IP:11739-1-AP, 4ug; Detection:11739-1-AP 1:2000) with mouse liver tissue lysate 4000 ug.

  • IF Staining of human liver cancer using 11739-1-AP

    IF Staining of human liver cancer using 11739-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ASGR1 antibody (11739-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human liver cancer using 11739-1-AP

    IF Staining of human liver cancer using 11739-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ASGR1 antibody (11739-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, human liver tissue, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inrat liver tissue, human liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue, human liver tissue, rat liver tissue
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ASGR1 Ready-To-Use IHC Kit. ASGR1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11739-1-AP targets ASGR1 in WB, IHC, IF-P, FC, IP, CoIP, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, FC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, FC, CoIP, Blocking assay
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Immunogen ASGR1 fusion protein Ag2310 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name asialoglycoprotein receptor 1
Synonyms asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1
Calculated Molecular Weight 291 aa, 33 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC032130
Gene Symbol ASGR1
Gene ID (NCBI) 432
RRIDAB_2059675
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07306
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

实验方案

Product Specific Protocols
WB protocol for ASGR1 antibody 11739-1-APDownload protocol
IHC protocol for ASGR1 antibody 11739-1-APDownload protocol
IF protocol for ASGR1 antibody 11739-1-APDownload protocol
IP protocol for ASGR1 antibody 11739-1-APDownload protocol
FC protocol for ASGR1 antibody 11739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseFC

Nature

Regulated control of gene therapies by drug-induced splicing.

Authors - Alex Mas Monteys
View Article
mouseFC

Nano Lett

A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo.

Authors - Khalid A Hajj
View Article
humanIF

Acta Biomater

Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply.

Authors - Kun Du
View Article
humanWB

Biomaterials

Dual targeted delivery of statins and nucleic acids by chitosan-based nanoparticles for enhanced antiatherosclerotic efficacy.

Authors - Ting Jiang
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humanWB

Cancer Res

ASO-Based PKM Splice-Switching Therapy Inhibits Hepatocellular Carcinoma Growth.

Authors - Wai Kit Ma
View Article
humanIHC,IF

Mol Ther

Enhanced Potency of GalNAc-Conjugated Antisense Oligonucleotides in Hepatocellular Cancer Models.

Authors - Youngsoo Kim
View Article