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ASGR1 Polyclonal antibody

ASGR1 Polyclonal Antibody for WB, IHC, IF-P, FC, IP, ELISA
Cat No. 11739-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF-P, FC, IP, ELISA and More (3)

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, human liver tissue, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inrat liver tissue, human liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue, human liver tissue, rat liver tissue
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ASGR1 Ready-To-Use IHC Kit. ASGR1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11739-1-AP targets ASGR1 in WB, IHC, IF-P, FC, IP, CoIP, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, FC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, FC, CoIP, Blocking assay
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, pig, monkey
免疫原 ASGR1 fusion protein Ag2310 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 asialoglycoprotein receptor 1
别名 asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1
计算分子量 291 aa, 33 kDa
观测分子量 38-42 kDa
GenBank蛋白编号BC032130
基因名称 ASGR1
Gene ID (NCBI) 432
RRIDAB_2059675
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07306
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

实验方案

Product Specific Protocols
WB protocol for ASGR1 antibody 11739-1-APDownload protocol
IHC protocol for ASGR1 antibody 11739-1-APDownload protocol
IF protocol for ASGR1 antibody 11739-1-APDownload protocol
IP protocol for ASGR1 antibody 11739-1-APDownload protocol
FC protocol for ASGR1 antibody 11739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseFC

Nature

Regulated control of gene therapies by drug-induced splicing.

Authors - Alex Mas Monteys
mouseFC

Nano Lett

A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo.

Authors - Khalid A Hajj
humanIF

Acta Biomater

Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply.

Authors - Kun Du
humanWB

Biomaterials

Dual targeted delivery of statins and nucleic acids by chitosan-based nanoparticles for enhanced antiatherosclerotic efficacy.

Authors - Ting Jiang
humanWB

Cancer Res

ASO-Based PKM Splice-Switching Therapy Inhibits Hepatocellular Carcinoma Growth.

Authors - Wai Kit Ma
humanIHC,IF

Mol Ther

Enhanced Potency of GalNAc-Conjugated Antisense Oligonucleotides in Hepatocellular Cancer Models.

Authors - Youngsoo Kim
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