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CoraLite® Plus 488-conjugated ASGR1 Polyclonal antibody

ASGR1 Polyclonal Antibody for IF-P, FC
Cat No. CL488-11739

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P, FC and More (1)

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF-P detected inrat liver tissue, human liver cancer tissue
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ASGR1 Ready-To-Use IHC Kit. ASGR1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11739-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-11739 targets ASGR1 in IF-P, FC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P, FC Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 ASGR1 fusion protein Ag2310 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 asialoglycoprotein receptor 1
别名 asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1
计算分子量 291 aa, 33 kDa
GenBank蛋白编号BC032130
基因名称 ASGR1
Gene ID (NCBI) 432
RRIDAB_2883080
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07306
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ASGR1 antibody CL488-11739Download protocol
FC protocol for CL Plus 488 ASGR1 antibody CL488-11739Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

MedComm (2020)

LILAR, a novel long noncoding RNA regulating autophagy in the liver tissues of endotoxemic mice through a competing endogenous RNA mechanism

Authors - Tian Tian
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