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验证数据展示

  • IF Staining of rat liver using CL555-11739

    IF Staining of rat liver using CL555-11739

    Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CoraLite®555 ASGR1 antibody (CL555-11739) at dilution of 1:200.

  • IF Staining of rat liver using CL555-11739

    IF Staining of rat liver using CL555-11739

    Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CoraLite®555 ASGR1 antibody (CL555-11739) at dilution of 1:200.

  • FC experiment of HepG2 using CL555-11739

    FC experiment of HepG2 using CL555-11739

    1X10^6 HepG2 cells were surface stained with 0.4 ug CoraLite®555 Anti-Human ASGR1 (CL555-11739) (red), or 0.4 ug Control Antibody. Cells were not fixed .

  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated ASGR1 Polyclonal antibody

ASGR1 Polyclonal Antibody for IF-P, FC
Cat No. CL555-11739

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC

Synonyms

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inrat liver tissue, human liver cancer tissue
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ASGR1 Ready-To-Use IHC Kit. ASGR1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11739-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-11739 targets ASGR1 in IF-P, FC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, FC Application Description
Tested Reactivity human, mouse, rat
Immunogen ASGR1 fusion protein Ag2310 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name asialoglycoprotein receptor 1
Synonyms asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1
Calculated Molecular Weight 291 aa, 33 kDa
GenBank Accession NumberBC032130
Gene Symbol ASGR1
Gene ID (NCBI) 432
RRIDAB_2919605
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07306
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

实验方案

Product Specific Protocols
IF protocol for CL555 ASGR1 antibody CL555-11739Download protocol
FC protocol for CL555 ASGR1 antibody CL555-11739Download protocol
Standard Protocols
Click here to view our Standard Protocols