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Acetyl-Histone H2B (Lys5) Recombinant antibody

Acetyl-Histone H2B (Lys5) Recombinant Antibody for WB, IF/ICC, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230539G10

Cat No : 83171-4-RR

Print datasheet

Synonyms

GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone cluster 2, H2be, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q

验证数据展示

  • WB analysis of NIH/3T3 using 83171-4-RR

    WB analysis of NIH/3T3 using 83171-4-RR

    Trichostatin A treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83171-4-RR (Acetyl-Histone H2B (Lys5) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83171-4-RR

    IF Staining of HeLa using 83171-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2B (Lys5) antibody (83171-4-RR, Clone: 230539G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • IF Staining of HeLa using 83171-4-RR

    IF Staining of HeLa using 83171-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2B (Lys5) antibody (83171-4-RR, Clone: 230539G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83171-4-RR

    FC experiment of HeLa using 83171-4-RR

    1x10^6 TSA treated HeLa cells were intracellularly stained with 0.25 ug Acetyl-Histone H2b (Lys5) Recombinant Antibody (83171-4-RR, Clone:230539G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive WB detected inTrichostatin A treated NIH/3T3 cells
Positive IF detected inHeLa cells
Positive FC detected inTSA treated HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83171-4-RR targets Acetyl-Histone H2B (Lys5) in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IF/ICC, FC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 2, H2be
Synonyms GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone cluster 2, H2be, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q
Calculated Molecular Weight 14 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC005827
Gene Symbol HIST2H2BE
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ16778
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Acetyl-Histone H2B (Lys5) antibody 83171-4-RRDownload protocol
IF protocol for Acetyl-Histone H2B (Lys5) antibody 83171-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols