• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated Acetyl-Histone H2B (Lys5) Recombinant antibody

Acetyl-Histone H2B (Lys5) Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230539G10

Cat no : CL488-83171-4

Print datasheet

Synonyms

HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1

验证数据展示

  • IF Staining of Hela using CL488-83171-4

    IF Staining of Hela using CL488-83171-4

    Immunofluorescent analysis of (4% PFA) fixed Hela cells using CoraLite® Plus 488 Acetyl-Histone H2B (Lys5) antibody (CL488-83171-4, Clone: 230539G10 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-83171-4

    FC experiment of HeLa using CL488-83171-4

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Acetyl-Histone H2b (Lys5) Recombinant Antibody (CL488-83171-4, Clone:230539G10)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive IF/ICC detected inHela cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 83171-4-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83171-4 targets Acetyl-Histone H2B (Lys5) in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 2, H2be
Synonyms HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1
Calculated Molecular Weight 14 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC005827
Gene Symbol Histone H2B
Gene ID (NCBI) 8349
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16778
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Acetyl-Histone H2B (Lys5) antibody CL488-83171-4Download protocol
Standard Protocols
Click here to view our Standard Protocols