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CoraLite® Plus 488-conjugated Alix Polyclonal antibody
Alix Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ALIX Ready-To-Use IHC Kit. ALIX primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12422-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-12422 targets Alix in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Alix fusion protein Ag3074 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | programmed cell death 6 interacting protein |
Synonyms | PDCD6IP, KIAA1375, Hp95, DRIP4, ALG-2-interacting protein X |
Calculated Molecular Weight | 868 aa, 96 kDa |
Observed Molecular Weight | 96 kDa, 75-80 kDa |
GenBank Accession Number | BC020066 |
Gene Symbol | Alix |
Gene ID (NCBI) | 10015 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8WUM4 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium. The alix antibody recognizes an additional band of 75-80 kDa which has also been observed in cells and exosomes.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 Alix antibody CL488-12422 | Download protocol |
FC protocol for CL Plus 488 Alix antibody CL488-12422 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |