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  • Featured Product
  • KD/KO Validated

Amphiregulin Polyclonal antibody, PBS Only

Amphiregulin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 16036-1-PBS

Print datasheet

Synonyms

AREG, AR, SDGF, CRDGF, Colorectum cell-derived growth factor

验证数据展示

  • WB analysis of MCF-7 using 16036-1-AP (same clone as 16036-1-PBS)

    WB analysis of MCF-7 using 16036-1-AP (same clone as 16036-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16036-1-AP (Amphiregulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 16036-1-AP (same clone as 16036-1-PBS)

    IHC staining of human stomach cancer using 16036-1-AP (same clone as 16036-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16036-1-AP (Amphiregulin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AREG antibody included
  • IHC staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

    IHC staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16036-1-AP (Amphiregulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AREG antibody included
  • IHC staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

    IHC staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16036-1-AP (Amphiregulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AREG antibody included
  • IP experiment of HepG2 using 16036-1-AP (same clone as 16036-1-PBS)

    IP experiment of HepG2 using 16036-1-AP (same clone as 16036-1-PBS)

    IP result of anti-Amphiregulin (IP:16036-1-AP, 4ug; Detection:16036-1-AP 1:2000) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 16036-1-AP (same clone as 16036-1-PBS)

    FC experiment of HeLa using 16036-1-AP (same clone as 16036-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Amphiregulin (16036-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

    IF Staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

    IF Staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AREG Ready-To-Use IHC Kit. AREG primary antibody included.

产品信息

16036-1-PBS targets Amphiregulin in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, FC (Intra), IP, ELISA Application Description
Tested Reactivity human
Immunogen Amphiregulin fusion protein Ag8907 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name amphiregulin
Synonyms AREG, AR, SDGF, CRDGF, Colorectum cell-derived growth factor
Calculated Molecular Weight 252 aa, 28 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC009799
Gene Symbol Amphiregulin/AREG
Gene ID (NCBI) 374
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15514
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms (PMID: 9642297).