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验证数据展示

  • WB analysis using 16036-1-AP

    WB analysis using 16036-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16036-1-AP (Amphiregulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 16036-1-AP

    WB analysis of MCF-7 using 16036-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16036-1-AP (Amphiregulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 16036-1-AP

    IHC staining of human stomach cancer using 16036-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16036-1-AP (Amphiregulin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AREG antibody included
  • IHC staining of human colon cancer using 16036-1-AP

    IHC staining of human colon cancer using 16036-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16036-1-AP (Amphiregulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AREG antibody included
  • IHC staining of human colon cancer using 16036-1-AP

    IHC staining of human colon cancer using 16036-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16036-1-AP (Amphiregulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AREG antibody included
  • IP experiment of HepG2 using 16036-1-AP

    IP experiment of HepG2 using 16036-1-AP

    IP result of anti-Amphiregulin (IP:16036-1-AP, 4ug; Detection:16036-1-AP 1:2000) with HepG2 cells lysate 3600ug.

  • IF Staining of mouse embryo using 16036-1-AP

    IF Staining of mouse embryo using 16036-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse embryo tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 16036-1-AP

    FC experiment of HeLa using 16036-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Amphiregulin (16036-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of human colon cancer using 16036-1-AP

    IF Staining of human colon cancer using 16036-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse embryo using 16036-1-AP

    IF Staining of mouse embryo using 16036-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse embryo tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human placenta using 16036-1-AP

    IF Staining of human placenta using 16036-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 16036-1-AP

    IF Staining of HepG2 using 16036-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Amphiregulin antibody (16036-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of human colon cancer using 16036-1-AP

    IF Staining of human colon cancer using 16036-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 16036-1-AP

    WB analysis using 16036-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16036-1-AP (Amphiregulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16036-1-AP

    IF Staining of HeLa using 16036-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

Amphiregulin Polyclonal antibody

Amphiregulin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 16036-1-AP

产品说明书

引用文献(38)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (1)

别名

AREG, AR, AREGB, Colorectum cell-derived growth factor, CRDGF

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, Calu-1 cells, HepG2 cells, L02 cells, HeLa cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman stomach cancer tissue, human colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue, mouse embryo tissue, human placenta tissue
Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy AREG Ready-To-Use IHC Kit. AREG primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16036-1-AP targets Amphiregulin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag8907

Product name: Recombinant human AREG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-252 aa of BC009799

Sequence: AGLDLNDTYSGKREPFSGDHSADGFEVTSRSEMSSGSEISPVSEMPSSSEPSSGADYDYSEEYDNEPQIPGYIVDDSVRVEQVVKPPQNKTESENTSDKPKRKKKGGKNGKNRRNRKKKNPCNAEFQNFCIHGECKYIEHLEAVTCKCQQEYFGERCGEKSMKTHSMIDSSLSKIALAAIAAFMSAVILTAVAVITVQLRRQYVRKYEGEAEERKKLRQENGNVHAIA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 amphiregulin
别名 AREG, AR, AREGB, Colorectum cell-derived growth factor, CRDGF
计算分子量 252 aa, 28 kDa
观测分子量 43-50 kDa
GenBank蛋白编号BC009799
基因名称 Amphiregulin/AREG
Gene ID (NCBI) 374
RRIDAB_2227602
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15514
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms (PMID: 9642297).

实验方案

Product Specific Protocols
FC protocol for Amphiregulin antibody 16036-1-APDownload protocol
IF protocol for Amphiregulin antibody 16036-1-APDownload protocol
IHC protocol for Amphiregulin antibody 16036-1-APDownload protocol
IP protocol for Amphiregulin antibody 16036-1-APDownload protocol
WB protocol for Amphiregulin antibody 16036-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nat Genet

Multi-omic analysis of gallbladder cancer identifies distinct tumor microenvironments associated with disease progression

Authors - Tao Zhou
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humanWB

Cancer Res

CD317 Activates EGFR by Regulating Its Association with Lipid Rafts.

Authors - Guizhong Zhang
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humanIHC

Adv Sci (Weinh)

Amphiregulin and Epiregulin Confer Radioresistance in Esophageal Squamous Cell Carcinoma Through Oxidative Phosphorylation.

Authors - Zhang Lin
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mouse

Biomaterials

EGCG-releasing nanofibrous scaffold enhances wound healing via γδ T cells modulation

Authors - Tiantian An
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rat,mouseWB

Cell Death Dis

O-GlcNAcylation of YAP1 promotes lung transplant ischemia-reperfusion injury via binding to HIF1α transcription factor and activating autophagy and mitophagy.

Authors - Shaohua Dai
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mouse

Cell Death Dis

TLR9 activation in large wound induces tissue repair and hair follicle regeneration via γδT cells

Authors - Xinhui Li
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