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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Amphiregulin Polyclonal antibody

Amphiregulin Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-16036

Print datasheet

Synonyms

amphiregulin, AR, AREG, AREGB, CRDGF, SDGF

验证数据展示

  • IF Staining of human colon cancer using CL647-16036

    IF Staining of human colon cancer using CL647-16036

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 647 Amphiregulin antibody (CL647-16036) at dilution of 1:200.

  • IF Staining of human colon cancer using CL647-16036

    IF Staining of human colon cancer using CL647-16036

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 647 Amphiregulin antibody (CL647-16036) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy AREG Ready-To-Use IHC Kit. AREG primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16036-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-16036 targets Amphiregulin in IF-P applications and shows reactivity with human samples.

Tested Applications IF-P Application Description
Tested Reactivity human
Immunogen Amphiregulin fusion protein Ag8907 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name amphiregulin
Synonyms amphiregulin, AR, AREG, AREGB, CRDGF, SDGF
Calculated Molecular Weight 252 aa, 28 kDa
GenBank Accession NumberBC009799
Gene Symbol Amphiregulin/AREG
Gene ID (NCBI) 374
RRIDAB_2920229
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15514
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms (PMID: 9642297).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Amphiregulin antibody CL647-16036Download protocol
Standard Protocols
Click here to view our Standard Protocols