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CoraLite® Plus 488-conjugated Amphiregulin Polyclonal antibody
Amphiregulin Polyclonal Antibody for IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF-P detected in | human colon cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy AREG Ready-To-Use IHC Kit. AREG primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16036-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-16036 targets Amphiregulin in IF-P applications and shows reactivity with human samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | human |
| Immunogen | Amphiregulin fusion protein Ag8907 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | amphiregulin |
| Synonyms | amphiregulin, AR, AREG, AREGB, CRDGF, SDGF |
| Calculated Molecular Weight | 252 aa, 28 kDa |
| GenBank Accession Number | BC009799 |
| Gene Symbol | Amphiregulin/AREG |
| Gene ID (NCBI) | 374 |
| RRID | AB_2919129 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15514 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms (PMID: 9642297).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Amphiregulin antibody CL488-16036 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |