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  • Featured Product
  • KD/KO Validated

BID Monoclonal antibody, PBS Only

BID Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A2E5

Cat no : 60301-1-PBS

Print datasheet

Synonyms

BID, FP497, p11 BID, p22 BID

验证数据展示

  • WB analysis of HeLa using 60301-1-Ig (same clone as 60301-1-PBS)

    WB analysis of HeLa using 60301-1-Ig (same clone as 60301-1-PBS)

    WB result of BID antibody (60301-1-Ig, 1:8000) with si-Control and si-BID transfected HeLa cells. This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 60301-1-Ig (same clone as 60301-1-PBS)

    WB analysis of HeLa using 60301-1-Ig (same clone as 60301-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60301-1-Ig (BID Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 60301-1-Ig (same clone as 60301-1-PBS)

    WB analysis of Jurkat using 60301-1-Ig (same clone as 60301-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60301-1-Ig (BID Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 60301-1-Ig (same clone as 60301-1-PBS)

    IHC staining of human colon cancer using 60301-1-Ig (same clone as 60301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60301-1-Ig (BID Antibody) at dilution of 1:900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BID antibody included
  • IHC staining of human colon cancer using 60301-1-Ig (same clone as 60301-1-PBS)

    IHC staining of human colon cancer using 60301-1-Ig (same clone as 60301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60301-1-Ig (BID Antibody) at dilution of 1:900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BID antibody included
  • IF Staining of HeLa using 60301-1-Ig (same clone as 60301-1-PBS)

    IF Staining of HeLa using 60301-1-Ig (same clone as 60301-1-PBS)

    Immunofluorescent analysis of HeLa cells using 60301-1-Ig (BID antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy BID Ready-To-Use IHC Kit. BID primary antibody included.

产品信息

60301-1-PBS targets BID in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen BID fusion protein Ag21005 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name BH3 interacting domain death agonist
Synonyms BID, FP497, p11 BID, p22 BID
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC009197
Gene Symbol BID
Gene ID (NCBI) 637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55957
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606).