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  • Featured Product
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BID Monoclonal antibody

BID Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A2E5

Cat no : 60301-1-Ig

Print datasheet

Synonyms

BID, FP497, p11 BID, p22 BID

验证数据展示

  • WB analysis of HeLa using 60301-1-Ig

    WB analysis of HeLa using 60301-1-Ig

    WB result of BID antibody (60301-1-Ig, 1:8000) with si-Control and si-BID transfected HeLa cells.

  • WB analysis of HeLa using 60301-1-Ig

    WB analysis of HeLa using 60301-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60301-1-Ig (BID Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 60301-1-Ig

    WB analysis of Jurkat using 60301-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60301-1-Ig (BID Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 60301-1-Ig

    IHC staining of human colon cancer using 60301-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60301-1-Ig (BID Antibody) at dilution of 1:900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BID antibody included
  • IHC staining of human colon cancer using 60301-1-Ig

    IHC staining of human colon cancer using 60301-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60301-1-Ig (BID Antibody) at dilution of 1:900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BID antibody included
  • IF Staining of HeLa using 60301-1-Ig

    IF Staining of HeLa using 60301-1-Ig

    Immunofluorescent analysis of HeLa cells using 60301-1-Ig (BID antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy BID Ready-To-Use IHC Kit. BID primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:1800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60301-1-Ig targets BID in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen BID fusion protein Ag21005 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name BH3 interacting domain death agonist
Synonyms BID, FP497, p11 BID, p22 BID
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC009197
Gene Symbol BID
Gene ID (NCBI) 637
RRIDAB_2881416
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55957
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606).

实验方案

Product Specific Protocols
WB protocol for BID antibody 60301-1-IgDownload protocol
IHC protocol for BID antibody 60301-1-IgDownload protocol
IF protocol for BID antibody 60301-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols