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验证数据展示

  • WB analysis using CL594-60301

    WB analysis using CL594-60301

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-60301 (BID antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated BID Monoclonal antibody

BID Monoclonal Antibody for WB
Cat No. CL594-60301

Print datasheet

CloneNo. 2A2E5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Synonyms

BID, FP497, p11 BID, p22 BID

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy BID Ready-To-Use IHC Kit. BID primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60301-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60301 targets BID in WB applications and shows reactivity with human samples.

Tested Applications WB Application Description
Tested Reactivity human
Immunogen BID fusion protein Ag21005 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name BH3 interacting domain death agonist
Synonyms BID, FP497, p11 BID, p22 BID
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC009197
Gene Symbol BID
Gene ID (NCBI) 637
RRIDAB_2919915
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP55957
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606).

实验方案

Product Specific Protocols
WB protocol for CL594 BID antibody CL594-60301Download protocol
Standard Protocols
Click here to view our Standard Protocols