验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy BID Ready-To-Use IHC Kit. BID primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60301-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-60301 targets BID in WB applications and shows reactivity with human samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | human |
| 免疫原 | BID fusion protein Ag21005 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | BH3 interacting domain death agonist |
| 别名 | BID, FP497, p11 BID, p22 BID |
| 计算分子量 | 22 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC009197 |
| 基因名称 | BID |
| Gene ID (NCBI) | 637 |
| RRID | AB_2919915 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P55957 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL594 BID antibody CL594-60301 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |